What is dust mites allergy

What is dust mites allergy

Allergen Details:

Allergen name: Der f 17
Lineage: Source: Animalia Arthropoda
Order: Astigmata
Species: Dermatophagoides farinae(American home dust mite)
Biochemical name: Calcium binding protein
MW(SDS-PAGE): 53 kDa
Allergenicity: As described in the publication, Der f 17 binds IgE in 35% of sera from mite-allergic patients.

The authors described obtaining a cDNA clone, but did not provide a sequence. Tategaki A, Kawamoto S, Aki T, Jyo T, Suzuki O, Shigeta S, Ono K. Newly described home dust mite allergens. ACI International suppl. 1:74-76, 2000

Route of allergen exposure: Airway
Date Created: 20-06-2006
Last Updated: 2019-08-15 01:26:42
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Table of IsoAllergens Click +/- for additional information

Isoallergen and variants GenBank Nucleotide GenBank Protein UniProt PDB
Der f 17.0101

Project 02

Susanne Vrtala, Dept.

of Pathophysiology and Allergy Research, Middle for Pathophysiology, Infectiology and Immunology, Medical University of Vienna.

Project 02: Strategies for diagnosis, treatment and prevention of home dust mite allergy.

Susanne Vrtala is working in the field of molecular allergy since 1990. Vrtala has received for her work regarding the molecular characterization of allergens and the engineering of recombinant hypoallergens for immunotherapy prestigious international awards such as the International Pharmacia and Allergopharma Awards. She and her group own worked on the characterization of home dust mite allergens.

Home dust mites (HDM) represent one of the most significant causes of allergy worldwide, against which more than 50% of allergic patients are sensitized. The characterization of the home dust mite allergens regarding their structural, immunological properties and especially regarding their clinical importance is an significant step for the development of new diagnostic tests, allergen-specific forms of immunotherapy and eventually prophylaxis. Home dust mites represent one of the most complicated allergen sources, containing several diverse allergens. The applicant will therefore first determine the importance of these allergen molecules using component-resolved diagnostic tests in diverse populations and study the allergenic activity and clinical relevance of the individual recombinant HDM allergens to identify those allergens which are necessary to be included into an effective vaccine for immunotherapy of mite allergic patients.

The clinically relevant allergens will be modified into hypoallergenic derivatives and linked in the form of hybrid proteins covering the clinically relevant allergen spectrum in the form of a few molecules to provide a feasible vaccination concept for home dust mite allergy. In vitro and in vivo evaluation of the hypoallergenic derivatives will identify the best candidate vaccines for immunotherapy of HDM allergic patients and eventually open possibilities for prophylactic vaccination. The project should thus ultimately lead to first immunotherapy trials in HDM allergic patients.

Assoc.Prof. Univ.-Doz. Dr. Susanne Vrtala
Department of Pathophysiology and Allergy Research
Waehringer Guertel 18-20
A 1090 Vienna, Austria
Tel: +43-1-40400 51320
Fax: +43-1-40400 51300
E-mail: This email address is being protected from spambots.

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Publications:

Weghofer, M., M. Grote, A. Casset, M. Kneidinger, Y. Resch, J. Kopec, W. R. Thomas, E. Fernández-Caldas, A. Mari, A. Purohit, G. Pauli, F. Horak, W. Keller, P. Valent, R.

What is dust mites allergy

Valenta, S. Vrtala. 2013. Identification of Der p 23, a peritrophin-like protein, as a new major Dermatophagoides pteronyssinus allergen associated with the peritrophic matrix of mite fecal pellets J. Immunol. In press.

Chen, K. W., K. Blatt, W. R. Thomas, I. Swoboda, P. Valent, R. Valenta, and S.

What is dust mites allergy

Vrtala. 2012. Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of home dust mite allergy. J. Allergy Clin. Immunol. 130: 435-443 e434.

Chen, K. W., M. Focke-Tejkl, K. Blatt, M. Kneidinger, A. Gieras, F. Dall’Antonia, I. Fae, G. Fischer, W. Keller, P. Valent, R. Valenta, and S. Vrtala. 2012. Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients. Allergy. 67: 609-621.

Resch, Y., M. Weghofer, S. Seiberler, F.

What is dust mites allergy

Horak, S. Scheiblhofer, B.

What is dust mites allergy

Linhart, I. Swoboda, W. R. Thomas, J. Thalhamer, R. Valenta, and S. Vrtala. 2011. Molecular characterization of Der p 10: a diagnostic marker for wide sensitization in home dust mite allergy. Clin. Exp. Allergy. 41: 1468-1477.

Weghofer, M., Y. Dall’Antonia, M. Grote, A. Stocklinger, M. Kneidinger, N. Balic, M. T. Krauth, E. Fernandez-Caldas, W. R. Thomas, M. van Hage, S. Vieths, S. Spitzauer, F. Horak, D. I. Svergun, P. V. Konarev, P. Valent, J. Thalhamer, W. Keller, R. Valenta, and S. Vrtala.

What is dust mites allergy

2008. Characterization of Der p 21, a new significant allergen derived from the gut of home dust mites. Allergy. 63: 758-767.

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Group References

  1. Johansson E; Aponno M; Lundberg M; van Hage-Hamsten MAllergenic cross-reactivity between the nematode Anisakis simplex and the dust mites Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Dermatophagoides pteronyssinus.Allergy.

    2001 Jul;56(7):660-6

  2. Eriksson TL; Whitley P; Johansson E; van Hage-Hamsten M; Gafvelin GIdentification and characterisation of two allergens from the dust mite Acarus siro, homologous with fatty acid-binding proteins.Int Arch Allergy Immunol. 1999 Aug;119(4):275-81
  3. Son M; Jeong KY; Kim BJ; Lim KJ; Lee JH; Park JWIgE reactivity to Acarus siro extract in Korean dust mite allergic patients.Exp Appl Acarol. 2014 May;63(1):57-64


Group Sequences

Species Common Allergen Type Length GI# Version
Acarus siro Mite Aca s 13 Aero Mite 131 118638268 9

After informed consent, subjects will be randomly assigned to ILIT group or placebo group in double-blind manner.

In both group, causal allergen or placebo will be injected into inguinal lymph node through guidance by ultrasonography three times with 4-week interval. In ILIT group, initial dose of allergen will be 1,000-fold diluted solution from maximal concentration of allergen extract for subcutaneous immunotherapy (Tyrosine S, Allergy Therapeutic, West Sussex, UK) in volume of 0.1ml. If skin is highly reactive in skin prick test, the initial dose will be 10-fold dilution from maximal concentration where diameter of wheal is less than that of histamine. After the first dose, allergen concentration will be escalated 3-fold at second dose, and 10-fold at third dose if there are no (or mild) local or systemic hypersensitivity reaction.

The allergen concentration will not change at second or third dose if there is moderate local or systemic reaction.

What is dust mites allergy

The allergen concentration will decrease by 10 or 100-fold from previous concentration or further injection will be held if there is severe local or systemic reaction after sufficient explanation and discussion with subjects.

The investigators will assess allergic rhinitis symptom score before and 4, 12 months after the initial treatment. Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ) and Sino-Nasal Outcome Test (SNOT-20) will be used. Visual analogue scale (VAS) of symptoms including rhinorrhea, sneezing, nasal obstruction, postnasal drip, eye/nose/ear/palate itching, dyspnea, wheezing, chest discomfort as well as urticaria, angioedema, and itching on exposed skin during exposure to causal allergen in daily life will be also evaluated.

Skin prick test (SPT), intradermal test (IDT), blood sampling for serum entire immunoglobulin E (IgE), allergen-specific IgE, and allergen-specific immunoglobulin G4 (IgG4), nasal lavage for Th1, Th2, and Treg cytokines, and nasal provocation test (NPT) with Df and/or Dp allergen (in subjects whose AR symptoms are provoked by Df and/or Dp) will be also performed before and 4, 12 months after the initial treatment. In addition, the investigators evaluated the change of subjects’ recognition of causal allergens, their avoidance, and AIT during this study. Using VAS, subjects were requested to score the rate of agreement with "Allergen provokes allergic symptoms in daily life", "Allergen avoidance can reduce allergic symptoms", "Allergen-specific Immunotherapy (AIT) can reduce allergic symptoms", "I can pay 50,000 Korean Won (KRW)/month for allergen avoidance", "I can pay 100,000 KRW/month for allergen avoidance", "I can pay 200,000 KRW/month for allergen avoidance", "I can pay 150,000 KRW for each injection of ILIT", "I can pay 300,000 KRW for each injection of ILIT", "I can pay 600,000 KRW for each injection of ILIT" before and after SPT/IDT, after NPT, 4 months and 1 year after ILIT.

Adverse events will be recorded and graded according to Muller classification and Ring and Meissner classification.


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